CD28 Protein (CD28) (AA 19-152) (Fc Tag,AVI tag,Biotin)
(1 reference)-
- 抗原 See all CD28 蛋白
- CD28
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 19-152
-
宿主
- 人, Cynomolgus, 猕猴
-
资源
- HEK-293 Cells
- 标记
- This CD28 protein is labelled with Fc Tag,AVI tag,Biotin.
- 品牌
- MABSol®,PrecisionAvi
- 序列
- AA 19-152
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.6 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product CD28 蛋白
-
CD28 (CD28) protein (mFc-His Tag)
CD28 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
CD28 (CD28) (AA 19-152) protein (His-Avi Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD28 (CD28) protein (His-Avi Tag,Biotin)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD28 (CD28) (AA 19-152) protein (Fc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (AA 19-152) protein (mFc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD28 (CD28) (AA 18-153) protein (mFc Tag)CD28 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (AA 18-153) protein (His tag)CD28 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (Active) protein (His tag)CD28 宿主: 人, Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD28 (CD28) (Active) protein (Biotin)CD28 宿主: 人, Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
-
- 说明
-
Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- Tris with Glycine, Arginine and NaCl, pH 7.5
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
-
-
: "Revealing the atomistic details behind the binding of B7-1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study." in: Biochimica et biophysica acta. General subjects, Vol. 1862, Issue 12, pp. 2764-2778, (2019) (PubMed).
-
: "Revealing the atomistic details behind the binding of B7-1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study." in: Biochimica et biophysica acta. General subjects, Vol. 1862, Issue 12, pp. 2764-2778, (2019) (PubMed).
-
- 抗原
- CD28
- 别名
- CD28 (CD28 产品)
- 别名
- Tp44 Protein, CD28RNA Protein, cd28 Protein, CD28 molecule Protein, CD28 antigen Protein, Cd28 molecule Protein, CD28 molecule L homeolog Protein, CD28 Protein, Cd28 Protein, cd28.L Protein
- 背景
- T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
- 分子量
- 43.6 kDa
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
-
