CD28 Protein (CD28) (AA 19-152) (Fc Tag,AVI tag,Biotin)
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Quick Overview for CD28 Protein (CD28) (AA 19-152) (Fc Tag,AVI tag,Biotin) (ABIN3137669)
抗原
See all CD28 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 19-152
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标记
- This CD28 protein is labelled with Fc Tag,AVI tag,Biotin.
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品牌
- MABSol® - PrecisionAvi
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序列
- AA 19-152
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特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
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产品特性
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This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.6 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE).
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization. -
内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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CD28 (CD28) (AA 19-152) (Active) protein (His-Avi Tag)
CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
CD28 (CD28) (AA 19-152) (Active) protein (Biotin,His-Avi Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
CD28 (CD28) (AA 19-152) protein (His tag,mFc Tag)CD28 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
CD28 (CD28) (AA 19-152) (Active) protein (hFc-DYKDDDDK Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (Active) protein (His tag)CD28 宿主: 人, Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD28 (CD28) (AA 19-152) (Active) protein (mFc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (AA 18-153) (Active) protein (His tag)CD28 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (AA 18-153) (Active) protein (mFc Tag)CD28 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (Active) protein (Biotin)CD28 宿主: 人, Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
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说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. -
限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Tris with Glycine, Arginine and NaCl, pH 7.5
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注意事项
- Please avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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: "Revealing the atomistic details behind the binding of B7-1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study." in: Biochimica et biophysica acta. General subjects, Vol. 1862, Issue 12, pp. 2764-2778, (2019) (PubMed).
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: "Revealing the atomistic details behind the binding of B7-1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study." in: Biochimica et biophysica acta. General subjects, Vol. 1862, Issue 12, pp. 2764-2778, (2019) (PubMed).
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- CD28
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别名
- CD28
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背景
- T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
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分子量
- 43.6 kDa
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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