ICOSLG Protein (AA 19-258) (His-Avi Tag)
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- 抗原 See all ICOSLG 蛋白
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
- 蛋白类型
- Recombinant
- 产品特性
- AA 19-258
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ICOSLG protein is labelled with His-Avi Tag.
- 原理
- Human B7-H2/ICOSLG Protein
- 序列
- Asp19-Ser258
- 产品特性
- Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human B7-H2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. The affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-258) protein (Fc Tag)
ICOSLG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) protein (His-Avi Tag,Biotin)ICOSLG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 47-279) protein (His tag)ICOSLG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-256) protein (His tag)ICOSLG 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 18-135) protein (His-DYKDDDDK-Strep II Tag)ICOSLG 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 18-256) protein (His-DYKDDDDK-Strep II Tag)ICOSLG 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) protein (mFc-His Tag)ICOSLG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. FACS
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 20-302) protein (GST tag)ICOSLG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (Active) protein (His tag,Fc Tag)ICOSLG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (Active) protein (His tag)ICOSLG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
- 别名
- B7-H2 (ICOSLG 产品)
- 别名
- ICOSLG Protein, AU044799 Protein, B7-H2 Protein, B7RP-1 Protein, B7h Protein, BG071784 Protein, GI50 Protein, GL50 Protein, GL50-B Protein, ICOS-L Protein, Icoslg Protein, LICOS Protein, Ly115l Protein, mKIAA0653 Protein, B7H2 Protein, B7RP1 Protein, CD275 Protein, ICOSL Protein, Icosl Protein, RGD1562791 Protein, inducible T-cell costimulator ligand Protein, icos ligand Protein, inducible T-cell co-stimulator ligand Protein, ICOSLG Protein, Icosl Protein, Icoslg Protein
- 背景
- B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion, induces also B-cell proliferation and differentiation into plasma cells.
- 分子量
- 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
- UniProt
- O75144
- 途径
- Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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