TNFSF9
(Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
蛋白类型
Recombinant
产品特性
Trimer
宿主
人
资源
HEK-293 Cells
标记
This TNFSF9 protein is labelled with Fc Tag,Biotin.
原理
Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)
序列
Arg71-Glu254
产品特性
Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Arg71-Glu254.
纯度
> 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human 4-1BB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer) , hFc Tag with the EC50 of 61ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
TNFSF9
宿主: 人
宿主: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22 μm filtered solution in PBS, 100 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFSF9
(Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.