TNFSF9
(Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
蛋白类型
Recombinant
产品特性
AA 68-251
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This TNFSF9 protein is labelled with Fc Tag.
原理
Cynomolgus 4-1BB Ligand/TNFSF9 Protein
序列
Arg68-Glu251
产品特性
Recombinant Cynomolgus 4-1BB Ligand/TNFSF9 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Arg68-Glu251.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus/Rhesus macaque 4-1BB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
TNFSF9
宿主: 人
宿主: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFSF9
(Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
分子量
46 kDa. Due to glycosylation, the protein migrates to 50-52 kDa based on Tris-Bis PAGE result.