BEBOV GP Protein (His tag)
BEBOV GP
宿主: Ebola Virus
宿主: Baculovirus infected Insect Cells
Recombinant
> 95 % as determined by SDS-PAGE.
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- 抗原 See all BEBOV GP products
- BEBOV GP (Bundibugyo Ebola Virus Envelope Glycoprotein (BEBOV GP))
- 蛋白类型
- Recombinant
- 宿主
- Ebola Virus
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资源
- Baculovirus infected Insect Cells
- 标记
- This BEBOV GP protein is labelled with His tag.
- 原理
- Recombinant EBOV (subtype Bundibugyo, strain Uganda 2007) GP1 / Glycoprotein Protein (His Tag)
- 序列
- Met1-Arg501
- 产品特性
- A DNA sequence encoding the Bundibugyo ebolavirus (strain Uganda 2007) GP (ACI28624.1) (Met1-Arg501) was expressed with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg protein as determined by the LAL method.
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Bundibugyo Ebola Virus Envelope Glycoprotein (BEBOV GP) protein (Fc Tag)
BEBOV GP 宿主: Ebola Virus 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- BEBOV GP (Bundibugyo Ebola Virus Envelope Glycoprotein (BEBOV GP))
- 别名
- BEBOV GP (BEBOV GP 产品)
- 背景
- The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
- 分子量
- 53.5kDa
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