SIGLEC15 Protein (mFc Tag)
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- 抗原 See all SIGLEC15 蛋白
- SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 人细胞
- 标记
- This SIGLEC15 protein is labelled with mFc Tag.
- 原理
- Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-mFC)
- 序列
- Phe19-Thr263
- 产品特性
- Recombinant Human Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr263 is expressed with a mFc tag at the C-terminus.
- 纯度
- >90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1 IEU/μg as determined by LAL test.
- Biological Activity Comment
- Immobilized Human Siglec-15-mFc (Cat#CW59) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 23.2 ng/ml.
- Top Product
- Discover our top product SIGLEC15 蛋白
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from a 0.2 μm filtered solution of PBS, 150 mM NaCl, 0.3 % Chaps, 5 % Trehalose, pH 7.4.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
- 别名
- Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (SIGLEC15 产品)
- 别名
- RGD1565948 Protein, CD33L3 Protein, HsT1361 Protein, SIGLEC-15 Protein, Cd33l3 Protein, EG620235 Protein, SIGLEC-I Protein, sialic acid binding Ig-like lectin 15 Protein, sialic acid binding Ig like lectin 15 Protein, Siglec15 Protein, SIGLEC15 Protein
- 背景
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Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts
Synonym: Angiopoietin-related protein 4, 425O18-1, Angiopoietin-like protein 4, Fasting-induced adipose factor, Hepatic fibrinogen/angiopoietin-related protein, HFARP, Secreted protein Bk89, Angptl4, Farp, Fiaf, Ng27
- 分子量
- 52.1 kDa
- UniProt
- Q6ZMC9
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