PPP3R1 Protein (His tag)
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- 抗原 See all PPP3R1 蛋白
- PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This PPP3R1 protein is labelled with His tag.
- 原理
- Recombinant Human PPP3R1 Protein (His Tag)(Active)
- 序列
- Gly2-Val170
- 产品特性
- A DNA sequence encoding the human PPP3R1 (P63098) (Gly2-Val170) was fused with a polyhistide tag at the N-terminus.
- 纯度
- > 87 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Using the Octet RED System, the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.
- Top Product
- Discover our top product PPP3R1 蛋白
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Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1) (AA 1-170) protein (GST tag)
PPP3R1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1) (AA 2-170) protein (His tag)Crystallography grade PPP3R1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1) (AA 2-170) protein (His tag)Crystallography grade PPP3R1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 2 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))
- 别名
- PPP3R1 (PPP3R1 产品)
- 别名
- si:ch211-274c22.8 Protein, canb Protein, CALNB1 Protein, CNB Protein, CNB1 Protein, CaNB1 Protein, Cnb1 Protein, MCIP1 Protein, protein phosphatase 3, regulatory subunit B, alpha a Protein, protein phosphatase 3 regulatory subunit B, alpha S homeolog Protein, protein phosphatase 3 regulatory subunit B, alpha Protein, protein phosphatase 3, regulatory subunit B, alpha Protein, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) Protein, ppp3r1a Protein, ppp3r1.S Protein, PPP3R1 Protein, Ppp3r1 Protein
- 背景
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Background: PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation, NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway, mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+)-pilocarpine, 2,4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway.
Synonym: Calcineurin Subunit B Type 1, Protein Phosphatase 2B Regulatory Subunit 1, Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1, PPP3R1, CNA2, CNB
- 分子量
- 21.4 kDa
- UniProt
- P63098
- 途径
- RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling
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