PPP3CA Protein (His tag)
-
- 抗原 See all PPP3CA 蛋白
- PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- Baculovirus infected Insect Cells
- 标记
- This PPP3CA protein is labelled with His tag.
- 原理
- Recombinant Human PPP3CA/CALNA Protein (His Tag)(Active)
- 序列
- Ser2-Gln521
- 产品特性
- A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.
- 纯度
- > 94 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Using the Octet RED System, the affinity constant (Kd) of human PPPP3CA-His bound to Human PPIA-His was 0.9 nM.
- Top Product
- Discover our top product PPP3CA 蛋白
-
Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA) (AA 1-84) protein (GST tag)
PPP3CA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA) (AA 1-511) protein (His tag)PPP3CA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA) (AA 2-521) protein (His tag)Crystallography grade PPP3CA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA) (AA 2-521) protein (His tag)Crystallography grade PPP3CA 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))
- 别名
- PPP3CA/CALNA (PPP3CA 产品)
- 别名
- CALN Protein, CALNA Protein, CALNA1 Protein, CCN1 Protein, CNA1 Protein, PPP2B Protein, 2900074D19Rik Protein, AI841391 Protein, AW413465 Protein, CN Protein, Caln Protein, Calna Protein, CnA Protein, Calna1 Protein, calcineurin Protein, caln Protein, calna Protein, calna1 Protein, ccn1 Protein, cna1 Protein, ppp2b Protein, fj46e08 Protein, si:dkeyp-79c2.2 Protein, wu:fj46e08 Protein, ppp3ca Protein, protein phosphatase 3 catalytic subunit alpha Protein, protein phosphatase 3, catalytic subunit, alpha isoform Protein, protein phosphatase 3, catalytic subunit, alpha isozyme Protein, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) Protein, protein phosphatase 3, catalytic subunit, alpha isozyme L homeolog Protein, serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Protein, PPP3CA Protein, Ppp3ca Protein, ppp3ca Protein, ppp3ca.L Protein, LOC100205420 Protein
- 背景
-
Background: PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.
Synonym: CALN,CALNA,CALNA1,CCN1,CNA1,PPP2B
- 分子量
- 60.8 kDa
- 途径
- RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling
-
