POLB Protein (His tag)
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- 抗原 See all POLB 蛋白
- POLB (Polymerase (DNA Directed), beta (POLB))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This POLB protein is labelled with His tag.
- 原理
- Recombinant Human DNA Polymerase β/POLB Protein (His Tag)
- 序列
- Ser2-Glu335
- 产品特性
- Recombinant Human DNA Polymerase beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product POLB 蛋白
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Polymerase (DNA Directed), beta (POLB) (AA 2-335) protein (His tag)
POLB 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)POLB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (GST tag)POLB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 224-333) protein (GST tag)POLB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (Strep Tag)Crystallography grade POLB 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (DNA Directed), beta (POLB) (Active) proteinPOLB 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure by SDS-PAGE analysis Func Active
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- POLB (Polymerase (DNA Directed), beta (POLB))
- 别名
- DNA Polymerase beta/POLB (POLB 产品)
- 别名
- wu:fa97a05 Protein, zgc:109924 Protein, POLB Protein, DDBDRAFT_0186152 Protein, DDBDRAFT_0215784 Protein, DDBDRAFT_0232376 Protein, DDB_0186152 Protein, DDB_0215784 Protein, DDB_0232376 Protein, A430088C08Rik Protein, polymerase (DNA directed), beta Protein, DNA polymerase beta Protein, DNA polymerase X family protein Protein, polymerase (DNA directed), beta S homeolog Protein, polb Protein, POLB Protein, polB Protein, polb.S Protein, Polb Protein
- 背景
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Background: Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.
Synonym: DNA Polymerase Beta, POLB
- 分子量
- 39.2 kDa
- UniProt
- P06746
- 途径
- DNA Damage Repair
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