POLB Protein (AA 2-335) (His tag)
-
- 抗原 See all POLB 蛋白
- POLB (Polymerase (DNA Directed), beta (POLB))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-335
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This POLB protein is labelled with His tag.
- 原理
- Recombinant Human DNA Polymerase β (C-6His)
- 序列
- MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSEVEHHH HHH
- 产品特性
- Recombinant Human DNA Polymerase beta is produced with our E. coli expression system. The target protein is expressed with sequence (Ser2-Glu335) of Human DNA Polymerase beta.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product POLB 蛋白
-
Polymerase (DNA Directed), beta (POLB) (AA 2-335) protein (His tag)
POLB 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)POLB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (GST tag)POLB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 224-333) protein (GST tag)POLB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (Strep Tag)Crystallography grade POLB 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (DNA Directed), beta (POLB) (Active) proteinPOLB 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure by SDS-PAGE analysis Func Active
-
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 溶解方式
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
-
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
-
- 抗原
- POLB (Polymerase (DNA Directed), beta (POLB))
- 别名
- DNA Polymerase beta (POLB 产品)
- 别名
- wu:fa97a05 Protein, zgc:109924 Protein, POLB Protein, DDBDRAFT_0186152 Protein, DDBDRAFT_0215784 Protein, DDBDRAFT_0232376 Protein, DDB_0186152 Protein, DDB_0215784 Protein, DDB_0232376 Protein, A430088C08Rik Protein, polymerase (DNA directed), beta Protein, DNA polymerase beta Protein, DNA polymerase X family protein Protein, polymerase (DNA directed), beta S homeolog Protein, polb Protein, POLB Protein, polB Protein, polb.S Protein, Polb Protein
- 背景
-
Human DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta.
Alternative Names: DNA Polymerase Beta, POLB - 分子量
- 39.23 kDa
- UniProt
- P06746
- 途径
- DNA Damage Repair
-
