PARP1 Protein (His tag)
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- 抗原 See all PARP1 蛋白
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This PARP1 protein is labelled with His tag.
- 原理
- Recombinant Mouse PARP-1 Protein (His Tag)(Active)
- 序列
- Met 1-Trp 1014
- 产品特性
- A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.
- 纯度
- > 85 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse PARP1 at 10 μg/mL (100 μl/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 μg/mL.
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- Discover our top product PARP1 蛋白
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Poly (ADP-Ribose) Polymerase 1 (PARP1) (Active) protein
PARP1 宿主: 人 宿主: Baculovirus Recombinant > 95 % WB, ELISA, PAR Active
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Internal Region) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Ct
Poly (ADP-Ribose) Polymerase 1 (PARP1) proteinPARP1 宿主: 人 宿主: Baculovirus Recombinant > 95 % WB, ELISA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (N-Term) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Ct
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 787-1014) protein (His-IF2DI Tag)PARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) protein (GST tag)PARP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 662-1014) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 2-1014) protein (His tag)Crystallography grade PARP1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 2-1013) protein (His tag)Crystallography grade PARP1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol, 0.1 mM TCEP
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- 别名
- PARP-1 (PARP1 产品)
- 别名
- ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein
- 背景
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Background: Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: 5830444G22Rik;Adprp;Adprt1;AI893648;ARTD1;C80510;PARP;parp-1;PPOL;sPARP-1
- 分子量
- 115 kDa
- NCBI登录号
- NP_031441
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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