PAI1 Protein (His tag)
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- 抗原 See all PAI1 (SERPINE1) 蛋白
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This PAI1 protein is labelled with His tag.
- 原理
- Recombinant Human SerpinE1/PAI-1 Protein (His Tag)(Active)
- 序列
- Met 1-Pro 402
- 产品特性
- A DNA sequence encoding the human SerpinE1 precursor (NP_000593.1) (Met 1-Pro 402) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 60 nM.
- Top Product
- Discover our top product SERPINE1 蛋白
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Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 24-402) protein (His tag)
SERPINE1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 1-402) protein (GST tag)SERPINE1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 24-402) protein (His tag)SERPINE1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 24-402) protein (His tag)SERPINE1 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 86-185) protein (GST tag)SERPINE1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Plasminogen Activator Inhibitor 1 (SERPINE1) protein (His tag)SERPINE1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM NaAc, 0.1M NaCl, pH 5.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
- 别名
- SerpinE1/PAI-1 (SERPINE1 产品)
- 别名
- PAI Protein, PAI-1 Protein, PAI1 Protein, PLANH1 Protein, SERPINE1 Protein, si:ch211-138a11.1 Protein, Planh1 Protein, PAI1A Protein, Pai1 Protein, Pai1aa Protein, Planh Protein, RATPAI1A Protein, serpin family E member 1 Protein, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog Protein, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 Protein, serine (or cysteine) peptidase inhibitor, clade E, member 1 Protein, SERPINE1 Protein, serpine1.L Protein, serpine1 Protein, Serpine1 Protein
- 背景
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Background: Plasminogen activator inhibitor 1, also known as PAI-1, Endothelial plasminogen activator inhibitor, SerpinE1 and PLANH1, is a secreted glycoprotein which belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SerpinE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma. High concentrations of SerpinE1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis. Studies of PAI-1 have contributed significantly to the elucidation of the protease inhibitory mechanism of serpins, which is based on a metastable native state becoming stabilised by insertion of the RCL into the central beta-sheet A and formation of covalent complexes with target proteases. Greater expression of PAI-1 has been associated with increased survival of cells and resistance to apoptosis. PAI-1 appears to influence apoptosis by decreasing cell adhesion (anoikis) as well as its effect on intracellular signaling. PAI-1, in its active state, also binds to the extracellular protein vitronectin. When in complex with its target proteases, it binds with high affinity to endocytosis receptors of the low density receptor family. The mechanisms of PAI-1 overexpression during obesity are complex, and it is conceivable that several inducers are involved at the same time at several sites of synthesis. PAI-1 is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis.
Synonym: Plasminogen Activator Inhibitor 1, PAI, PAI-1, Endothelial Plasminogen Activator Inhibitor, Serpin E1, SERPINE1, PAI1, PLANH1,SERPINE1
- 分子量
- 44.2 kDa
- NCBI登录号
- NP_000593
- 途径
- p53 Pathway, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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