MAPKAP Kinase 5 Protein (GST tag,His tag)
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- 抗原 See all MAPKAP Kinase 5 (MAPKAPK5) 蛋白
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This MAPKAP Kinase 5 protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human MAPKAPK5 Protein (His & GST Tag)
- 序列
- Met 1-Gln 471
- 产品特性
- A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 76 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product MAPKAPK5 蛋白
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Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) protein (GST tag)
MAPKAPK5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA, KAA
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) (Active) protein (GST tag)MAPKAPK5 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) (Active) proteinMAPKAPK5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 371-471) protein (GST tag)MAPKAPK5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-473) protein (Strep Tag)Crystallography grade MAPKAPK5 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-473) protein (Strep Tag)Crystallography grade MAPKAPK5 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) protein (His tag)MAPKAPK5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
- 别名
- MAPKAPK5 (MAPKAPK5 产品)
- 别名
- MAPKAPK5 Protein, MK5 Protein, PRAK Protein, MAPKAP-K5 Protein, MK-5 Protein, mitogen-activated protein kinase-activated protein kinase 5 Protein, MAP kinase-activated protein kinase 5 Protein, MAPKAPK5 Protein, mapkapk5 Protein, Mapkapk5 Protein
- 背景
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Background: MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.
Synonym: MAPKAP-K5,MK-5,MK5,PRAK
- 分子量
- 82 kDa
- NCBI登录号
- NP_003659
- 途径
- MAPK Pathway
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