GADD45A Protein (GST tag,His tag)
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- 抗原 See all GADD45A 蛋白
- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This GADD45A protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human GADD45A/DDIT-1 Protein (His & GST Tag)
- 序列
- Met 1-Arg 165
- 产品特性
- A DNA sequence encoding the full length of human GADD45A (P24522) (Met 1-Arg 165) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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- Discover our top product GADD45A 蛋白
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Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (GST tag)
GADD45A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 76-165) protein (GST tag)GADD45A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (Strep Tag)Crystallography grade GADD45A 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (Strep Tag)Crystallography grade GADD45A 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165), (full length) protein (His-SUMO Tag)GADD45A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) protein (Myc-DYKDDDDK Tag)GADD45A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
- 别名
- GADD45A/DDIT-1 (GADD45A 产品)
- 别名
- MGC53491 Protein, zgc:91795 Protein, gadd45a Protein, GADD45A Protein, ddit1 Protein, gadd45 Protein, GADD45 Protein, AA545191 Protein, Ddit1 Protein, DDIT1 Protein, Gadd45 Protein, growth arrest and DNA damage inducible alpha L homeolog Protein, growth arrest and DNA-damage-inducible, alpha, b Protein, growth arrest and DNA damage inducible alpha Protein, growth arrest and DNA damage-inducible protein 45 Protein, growth arrest and DNA-damage-inducible 45 alpha Protein, growth arrest and DNA-damage-inducible, alpha Protein, gadd45a.L Protein, gadd45ab Protein, GADD45A Protein, gadd45a Protein, GADD45 Protein, Gadd45a Protein
- 背景
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Background: GADD45A is a member of the GADD45 Family, and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions, including MAPK signaling, cell cycle regulation, DNA repair and genomic stability, apoptosis, and immune responses. The GADD45 Family of genes is rapidly induced by different stressors, including differentiation-inducing cytokines, and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins, including Cdc2 protein kinase, proliferating cell nuclear antigen (PCNA), p21Waf1/Cip1 protein, core histone protein and MTK/MEKK4, an up-stream activator of the JNK/SAPK pathway, indicating that GADD45A may play important roles in the control of cell cycle checkpoint, DNA repair process, and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario, wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues, p63 and p73. The decreased GADD45A expression is also considered a survival mechanism, as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells, it presents itself as an emerging drug target worthy of further investigation.
Synonym: Growth Arrest and DNA Damage-Inducible Protein GADD45 Alpha, DNA Damage-Inducible Transcript 1 Protein, DDIT-1, GADD45A, DDIT1, GADD45
- 分子量
- 46.2 kDa
- UniProt
- P24522
- 途径
- p53 Pathway, Cell Division Cycle
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