FH Protein (His tag)
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- 抗原 See all FH 蛋白
- FH (Fumarate Hydratase (FH))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This FH protein is labelled with His tag.
- 原理
- Recombinant Human Fumarase/FH Protein (His Tag)(Active)
- 序列
- Ala 45-Lys 510
- 产品特性
- A DNA sequence encoding the mature form of human FH (P07954-1) (Ala 45-Lys 510) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
- 纯度
- > 85 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its ability to transform 1umole of Fumarate to L-malate per minute at pH 7.5 at 37!aC. Specific activity is > 25 unit/mg
- Top Product
- Discover our top product FH 蛋白
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Fumarate Hydratase (FH) (AA 44-510) protein
FH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Fumarate Hydratase (FH) (AA 33-510) protein (GST tag)FH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fumarate Hydratase (FH) (AA 45-510) protein (His tag)Crystallography grade FH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fumarate Hydratase (FH) (AA 42-507) protein (His tag)Crystallography grade FH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fumarate Hydratase (FH) protein (Myc-DYKDDDDK Tag)FH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 10 mM Tris, 5 mM EDTA, 1 mM DTT, pH 7.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- FH (Fumarate Hydratase (FH))
- 别名
- Fumarase/FH (FH 产品)
- 别名
- FH Protein, DDBDRAFT_0205237 Protein, DDBDRAFT_0231397 Protein, DDBDRAFT_0231400 Protein, DDB_0205237 Protein, DDB_0231397 Protein, DDB_0231400 Protein, HLRCC Protein, LRCC Protein, MCL Protein, MCUL1 Protein, Fh1 Protein, im:7152785 Protein, ns:zf-e152 Protein, zf-e152 Protein, zgc:66253 Protein, zgc:77498 Protein, Fh Protein, Fh-1 Protein, fumarate hydratase Protein, fumarate hydratase 1 Protein, FH Protein, fumH Protein, Fh Protein, fh Protein, Fh1 Protein
- 背景
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Background: Fumarate Hydratase (FH) is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. Fumarate Hydratase is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Individuals with hemizygous germline fumarate hydratase (FH) mutations are predisposed to renal cancer. These tumors predominantly exhibit functional inactivation of the remaining wild-type allele, implicating FH inactivation as a tumor-promoting event.
Synonym: Fumarate Hydratase Mitochondrial, Fumarase, FH,FMRD,HLRCC,LRCC,MCL,MCUL1
- 分子量
- 52 kDa
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