FH Protein (AA 45-510) (His tag)
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- 抗原 See all FH 蛋白
- FH (Fumarate Hydratase (FH))
- 蛋白类型
- Recombinant
- 产品特性
- AA 45-510
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This FH protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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ASQNSFRIEY DTFGELKVPN DKYYGAQTVR STMNFKIGGV TERMPTPVIK AFGILKRAAA EVNQDYGLDP KIANAIMKAA DEVAEGKLND HFPLVVWQTG SGTQTNMNVN EVISNRAIEM LGGELGSKIP VHPNDHVNKS QSSNDTFPTA MHIAAAIEVH EVLLPGLQKL HDALDAKSKE FAQIIKIGRT HTQDAVPLTL GQEFSGYVQQ VKYAMTRIKA AMPRIYELAA GGTAVGTGLN TRIGFAEKVA AKVAALTGLP FVTAPNKFEA LAAHDALVEL SGAMNTTACS LMKIANDIRF LGSGPRSGLG ELILPENEPG SSIMPGKVNP TQCEAMTMVA AQVMGNHVAV TVGGSNGHFE LNVFKPMMIK NVLHSARLLG DASVSFTENC VVGIQANTER INKLMNESLM LVTALNPHIG YDKAAKIAKT AHKNGSTLKE TAIELGYLTA EQFDEWVKPK DMLGPK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human FH Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product FH 蛋白
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Fumarate Hydratase (FH) (AA 44-510) protein
FH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Fumarate Hydratase (FH) (AA 33-510) protein (GST tag)FH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fumarate Hydratase (FH) (AA 42-507) protein (His tag)Crystallography grade FH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fumarate Hydratase (FH) protein (Myc-DYKDDDDK Tag)FH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- FH (Fumarate Hydratase (FH))
- 别名
- FH (FH 产品)
- 别名
- FH Protein, DDBDRAFT_0205237 Protein, DDBDRAFT_0231397 Protein, DDBDRAFT_0231400 Protein, DDB_0205237 Protein, DDB_0231397 Protein, DDB_0231400 Protein, HLRCC Protein, LRCC Protein, MCL Protein, MCUL1 Protein, Fh1 Protein, im:7152785 Protein, ns:zf-e152 Protein, zf-e152 Protein, zgc:66253 Protein, zgc:77498 Protein, Fh Protein, Fh-1 Protein, fumarate hydratase Protein, fumarate hydratase 1 Protein, FH Protein, fumH Protein, Fh Protein, fh Protein, Fh1 Protein
- 背景
- Also acts as a tumor suppressor.
- 分子量
- 51.0 kDa Including tag.
- UniProt
- P07954
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