NEIL1 Protein (His tag)
-
- 抗原 See all NEIL1 蛋白
- NEIL1 (Endonuclease VIII-Like 1 (NEIL1))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This NEIL1 protein is labelled with His tag.
- 原理
- Recombinant Human NEIL1 Protein (His Tag)
- 序列
- Met 1-Ser 390
- 产品特性
- A DNA sequence encoding the human NEIL1 (AAH10876.1) (Met 1-Ser 390) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
- 纯度
- > 84 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product NEIL1 蛋白
-
Endonuclease VIII-Like 1 (NEIL1) (AA 302-390) protein (GST tag)
NEIL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Endonuclease VIII-Like 1 (NEIL1) (AA 200-390) protein (His tag)NEIL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Endonuclease VIII-Like 1 (NEIL1) (AA 1-390) protein (His tag)NEIL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Endonuclease VIII-Like 1 (NEIL1) (AA 1-390) protein (GST tag)NEIL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Endonuclease VIII-Like 1 (NEIL1) (AA 2-390) protein (His-SUMO Tag)NEIL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Endonuclease VIII-Like 1 (NEIL1) protein (Myc-DYKDDDDK Tag)NEIL1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- NEIL1 (Endonuclease VIII-Like 1 (NEIL1))
- 别名
- NEIL1 (NEIL1 产品)
- 别名
- FPG1 Protein, NEI1 Protein, hFPG1 Protein, 2810450N13Rik Protein, Nei1 Protein, nei-like DNA glycosylase 1 Protein, nei like DNA glycosylase 1 Protein, nei endonuclease VIII-like 1 (E. coli) Protein, Neil1 Protein, NEIL1 Protein
- 背景
-
Background: NEIL1 is a member of DNA glycosylases. DNA glycosylases are a family homologous to the bacterial Fpg/Nei family. They play a role in base excision repair which is the mechanism by which damaged bases in DNA are removed and replaced. The first step of this process is catalyzed by DNA glycosylases. They remove the damaged nitrogenous base while leaving the sugar-phosphate backbone intact, creating an apurinic/apyrimidinic site, commonly referred to as an AP site. NEIL1 functions in base excision repair of DNA damaged by oxidation or by mutagenic agents. It acts as DNA glycosylase that recognizes and removes damaged bases. NEIL1 prefers to oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand and cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
Synonym: FPG1,hFPG1,NEI1
- 分子量
- 45 kDa
- 途径
- DNA Damage Repair
-
