CSTB Protein (His tag)
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- 抗原 See all CSTB 蛋白
- CSTB (Cystatin B (Stefin B) (CSTB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CSTB protein is labelled with His tag.
- 原理
- Recombinant Human Cystatin B/CSTB Protein (His Tag)(Active)
- 序列
- Met 2-Phe 98
- 产品特性
- A DNA sequence encoding the human CSTB (P04080) (Met 2-Phe 98) was expressed, with a polyhistide tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (R&D Systems, Catalog # ES009). The IC50 value is < 15 nM.
- Top Product
- Discover our top product CSTB 蛋白
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Cystatin B (Stefin B) (CSTB) (AA 1-98) protein (GST tag)
CSTB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cystatin B (Stefin B) (CSTB) (AA 1-98), (full length) protein (GST tag)CSTB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % SDS, WB, ELISA
Cystatin B (Stefin B) (CSTB) (AA 1-98) (Active) protein (His tag)CSTB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS Active
Cystatin B (Stefin B) (CSTB) proteinCSTB 宿主: Cow 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
Cystatin B (Stefin B) (CSTB) protein (Myc-DYKDDDDK Tag)CSTB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 50 mM NaCl, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CSTB (Cystatin B (Stefin B) (CSTB))
- 别名
- Cystatin B/CSTB (CSTB 产品)
- 别名
- CST6 Protein, EPM1 Protein, EPM1A Protein, PME Protein, STFB Protein, ULD Protein, CSTB Protein, Cyb Protein, Epm1 Protein, Stfb Protein, MGC189005 Protein, pme Protein, cst6 Protein, epm1 Protein, stfb Protein, AA960480 Protein, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Protein, ATCYS6 Protein, ATCYSB Protein, cystatin B Protein, LOC100008600 Protein, LOC100136235 Protein, CPI1 Protein, cystatin B Protein, cystatin B (stefin B) Protein, cystatin 14a, tandem duplicate 2 Protein, cystatin-B Protein, CSTB Protein, Cstb Protein, cstb Protein, cst14a.2 Protein, CYSB Protein, cpi1 Protein, LOC101123265 Protein
- 背景
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Background: Cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to the cystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).
Synonym: CST6;EPM1;EPM1A;PME;STFB;ULD
- 分子量
- 12.5 kDa
- UniProt
- P04080
- 途径
- Response to Water Deprivation
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