CSTB Protein (AA 1-98) (His tag)
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- 抗原 See all CSTB 蛋白
- CSTB (Cystatin B (Stefin B) (CSTB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-98
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CSTB protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
- Top Product
- Discover our top product CSTB 蛋白
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Cystatin B (Stefin B) (CSTB) (AA 1-98) protein (GST tag)
CSTB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cystatin B (Stefin B) (CSTB) (AA 1-98) protein (Strep Tag)Crystallography grade CSTB 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Cystatin B (Stefin B) (CSTB) (AA 1-98), (full length) protein (GST tag)CSTB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % SDS, WB, ELISA
Cystatin B (Stefin B) (CSTB) proteinCSTB 宿主: Cow 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
Cystatin B (Stefin B) (CSTB) protein (Myc-DYKDDDDK Tag)CSTB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 50 mM NaCl
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- CSTB (Cystatin B (Stefin B) (CSTB))
- 别名
- CSTB (CSTB 产品)
- 别名
- CST6 Protein, EPM1 Protein, EPM1A Protein, PME Protein, STFB Protein, ULD Protein, CSTB Protein, Cyb Protein, Epm1 Protein, Stfb Protein, MGC189005 Protein, pme Protein, cst6 Protein, epm1 Protein, stfb Protein, AA960480 Protein, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Protein, ATCYS6 Protein, ATCYSB Protein, cystatin B Protein, LOC100008600 Protein, LOC100136235 Protein, CPI1 Protein, cystatin B Protein, cystatin B (stefin B) Protein, cystatin 14a, tandem duplicate 2 Protein, cystatin-B Protein, CSTB Protein, Cstb Protein, cstb Protein, cst14a.2 Protein, CYSB Protein, cpi1 Protein, LOC101123265 Protein
- 背景
- CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus. Recombinant CSTB, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 13 kDa (118aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_000091
- UniProt
- P04080
- 途径
- Response to Water Deprivation
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