Elastase 4 Protein (His tag)
-
- 抗原 See all Elastase 4 (CTRC) products
- Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This Elastase 4 protein is labelled with His tag.
- 原理
- Recombinant Human Chymotrypsin C Protein (His Tag)(Active)
- 序列
- Met 1-Leu 268
- 产品特性
- A DNA sequence encoding the human CTRC (Q99895) (Met 1-Leu 268) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate, SUC-Ala-Ala-Pro-Phe-AMC.The specific activity is >300 pmol/min/μg.
-
Chymotrypsin C (Caldecrin) (CTRC) (AA 1-268) protein (GST tag)
CTRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chymotrypsin C (Caldecrin) (CTRC) (AA 170-268) protein (GST tag)CTRC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chymotrypsin C (Caldecrin) (CTRC) proteinCTRC 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))
- 别名
- Chymotrypsin C (CTRC 产品)
- 别名
- CLCR Protein, ELA4 Protein, 1810044E12Rik Protein, bPTLP Protein, chymotrypsin C Protein, chymotrypsin-C Protein, chymotrypsin C (caldecrin) Protein, CTRC Protein, LOC478220 Protein, Ctrc Protein
- 背景
-
Background: Chymotrypsin C (abbreviated for CTRC); also known as caldecrin or elastase4; is a digestive enzyme of the peptidase S1 family. This enzyme is synthesized as an inactivate chymotrypsinogen. On cleavage by trypsin into two parts that activate each other by removing two small peptides in a trans-proteolysis; chymotrypsin C produced. N-linked glycosylation of human CTRC is required for efficient folding and secretion; however; the N-linked glycan is unimportant for enzyme activity or inhibitor binding. It has been proposed that CTRC is a key regulator of digestive zymogen activation and a physiological co-activator of digestive carboxypeptidases proCPA1 and proCPA2. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis. It's speculated that CTRC might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. The pancreatic cancer cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed CTRC; whereas the cell migration ability was upregulated in Aspc-1 cells in which CTRC was suppressed.
Synonym: Chymotrypsin-C; Caldecrin; CTRC; CLCR
- 分子量
- 29.3 kDa
- UniProt
- Q99895
-
