SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (BA.2 - Omicron, BA.3 - Omicron) (His-Avi Tag,Biotin)
-
- 抗原 See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 蛋白
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 蛋白类型
- Recombinant
- 产品特性
- BA.2 - Omicron, BA.3 - Omicron
-
宿主
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron
-
资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Nucleocapsid protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron)
- 序列
- Met 1 - Ala 419
- 产品特性
- Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P13L, ERS31-33del, R203K, G204R, S413R). The nucleocapsid mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2, GISAID clade: GRA, Nextstrain clade: 21L & Pango lineage: BA.3, GISAID clade: GRA, Nextstrain clade: 21M).
- 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 N 蛋白
-
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein
SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: Mammalian Cells Recombinant Greater than 85 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) protein (His tag,AVI tag,Biotin)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. ELISA, SDS
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 243-364) protein (His-Avi Tag,Biotin)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 64-180) protein (His-Avi Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: CHO Cells Recombinant >95 % as determined by SDS-PAGE and HPLC
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His-SUMOstar Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 酵母菌 Recombinant Greater than 90 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His-PDI Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 酵母菌 Recombinant Greater than 85 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (G204R), (R203K) (Active) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
-
- 应用备注
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 48.8 kDa. The protein migrates as 33 kDa and 50-60 kDa under reducing (R) condition .
- 说明
-
Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- PBS, 0.2M Arginine, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- -20°C
-
- 抗原
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 别名
- SARS-CoV-2 Nucleocapsid protein (SARS-CoV-2 N 产品)
- 别名
- nucleocapsid phosphoprotein Protein, N Protein
- 物质类
- Viral Protein
- 背景
-
Synonyms: Nucleocapsid protein,NP,Protein N,
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool. - 分子量
- 48.8 kDa
-
