SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag,AVI tag,Biotin)
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- 抗原 See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 蛋白
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 蛋白类型
- Recombinant
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宿主
- SARS Coronavirus-2 (SARS-CoV-2)
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SARS-CoV-2 Nucleocapsid protein is labelled with His tag,AVI tag,Biotin.
- 应用范围
- SDS-PAGE (SDS), ELISA
- 原理
- Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™
- 序列
- AA 1-419
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
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Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag is expressed from E.coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). - 纯度
- >90 % as determined by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 N 蛋白
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SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein
SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: Mammalian Cells Recombinant Greater than 85 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 243-364) protein (His-Avi Tag,Biotin)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 64-180) protein (His-Avi Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: CHO Cells Recombinant >95 % as determined by SDS-PAGE and HPLC
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His-SUMOstar Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 酵母菌 Recombinant Greater than 90 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His-PDI Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 酵母菌 Recombinant Greater than 85 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (G204R), (R203K) (Active) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (T205I) (Active) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 50 mM Tris, 150 mM NaCl, Arginine, pH 7.5
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.
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: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).
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: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).
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- 抗原
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 别名
- SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N 产品)
- 别名
- nucleocapsid phosphoprotein Protein, N Protein
- 物质类
- Viral Protein
- 背景
- Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
- 分子量
- 51.1 kDa
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