Claudin 18.2 (Active) protein (His-Avi Tag,Biotin)
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- 抗原
- Claudin 18.2
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Insect Cells
- 标记
- His-Avi Tag,Biotin
- 原理
- Biotinylated Human Claudin-18.2 Protein, His,Avitag™ (active membrane protein)
- 序列
- Met 1 - Ala 200
- 产品特性
- Biotinylated Human Claudin-18.2, His,Avitag (CL2-H82E3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2).
- 纯度
- >60 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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Claudin 18.2 (AA 1-261) (Active) protein-VLP
宿主: 人 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC ELISA, Func, Imm, SPR Active
Claudin 18.2 (AA 1-261) (Active) protein-VLP (Biotin)宿主: 人 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC ELISA, Func, Imm, SPR Active
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- 应用备注
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This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.8 kDa.
It is produced as active membrane protein with full ECD epitopes, and contains both the ECL1 and ECL2. - 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH 7.5
- 储存条件
- -80 °C
- 储存方法
- -70°C
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- 抗原
- Claudin 18.2
- 别名
- Claudin-18.2
- 背景
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Synonyms: Claudin-18.2,CLDN18,Claudin-18,
Description: Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells. - 分子量
- 24.8 kDa
- NCBI登录号
- NP_001002026
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