SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag)
(5 references)-
- 抗原 See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 蛋白
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 蛋白类型
- Recombinant
-
宿主
- SARS Coronavirus-2 (SARS-CoV-2)
-
资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.
- 序列
- AA 1-419
- 产品特性
- 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag (NUN-C5227) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # YP_009724397.2). This protein carries a polyhistidine tag at the C-terminus.
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 N 蛋白
-
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein
SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: Mammalian Cells Recombinant Greater than 85 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) protein (His tag,AVI tag,Biotin)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. SDS, ELISA
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 243-364) protein (His-Avi Tag,Biotin)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 64-180) protein (His-Avi Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: CHO Cells Recombinant >95 % as determined by SDS-PAGE and HPLC
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His-SUMOstar Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 酵母菌 Recombinant Greater than 90 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) protein (His-PDI Tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 酵母菌 Recombinant Greater than 85 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE.
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (G204R), (R203K) (Active) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (T205I) (Active) protein (His tag)SARS-CoV-2 N 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
-
- 应用备注
- The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition due to glycosylation.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
-
-
: "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 5, (2021) (PubMed).
: "Variable post-translational modifications of SARS-CoV-2 nucleocapsid protein." in: Glycobiology, (2021) (PubMed).
: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).
: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
: "SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5064, (2020) (PubMed).
-
: "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 5, (2021) (PubMed).
-
- 抗原
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 别名
- SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N 产品)
- 别名
- nucleocapsid phosphoprotein Protein, N Protein
- 物质类
- Viral Protein
- 背景
- Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
- 分子量
- 47.3 kDa
-
