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CD25 Protein (AA 22-213) (His tag,AVI tag,Biotin)

IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
产品编号 ABIN6731243
发货至: 中国
  • 抗原 See all CD25 (IL2RA) 蛋白
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 22-213
    宿主
    • 22
    • 11
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    资源
    • 14
    • 10
    • 8
    • 5
    • 4
    • 3
    • 1
    • 1
    HEK-293 Cells
    标记
    This CD25 protein is labelled with His tag,AVI tag,Biotin.
    序列
    AA 22-213
    特异性
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    纯度
    >90 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product IL2RA 蛋白
  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
  • 抗原
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    别名
    IL-2 R alpha (IL2RA 产品)
    别名
    CD25 Protein, IDDM10 Protein, IL2R Protein, TCGFR Protein, IL2RAC Protein, IL-2RA Protein, Il2r Protein, Ly-43 Protein, interleukin 2 receptor subunit alpha Protein, interleukin-2 receptor subunit alpha-like Protein, interleukin 2 receptor, alpha chain Protein, IL2RA Protein, Il2ra Protein, LOC100222957 Protein
    背景
    Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
    分子量
    25.4 kDa
    NCBI登录号
    NP_000408
    途径
    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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