CD25 Protein (AA 22-236) (His tag)
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- 抗原 See all CD25 (IL2RA) 蛋白
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-236
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This CD25 protein is labelled with His tag.
- 原理
- Mouse IL-2 R alpha/CD25 Protein
- 序列
- Glu22-Lys236
- 产品特性
- Recombinant Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse IL-2 R alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 95.8ng/ml determined by ELISA. The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His-Avi Tag)
IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His-Avi Tag,Biotin)IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (Fc Tag)IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) (Active) protein (His tag)IL2RA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 223-798) proteinIL2RA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Interleukin 2 Receptor, alpha (IL2RA) protein (His tag)IL2RA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-240) protein (His tag)Crystallography grade IL2RA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) protein (His tag)Crystallography grade IL2RA 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-272) protein (rho-1D4 tag)Crystallography grade IL2RA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-268) protein (rho-1D4 tag)Crystallography grade IL2RA 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 别名
- IL-2 R alpha (IL2RA 产品)
- 别名
- CD25 Protein, IDDM10 Protein, IL2R Protein, TCGFR Protein, IL2RAC Protein, IL-2RA Protein, Il2r Protein, Ly-43 Protein, interleukin 2 receptor subunit alpha Protein, interleukin-2 receptor subunit alpha-like Protein, interleukin 2 receptor, alpha chain Protein, IL2RA Protein, Il2ra Protein, LOC100222957 Protein
- 背景
- IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
- 分子量
- 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P01590
- 途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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