PPM1G Protein (AA 1-546) (His tag)
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- 抗原 See all PPM1G 蛋白
- PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-546
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PPM1G protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
- 纯度
- > 85 % by SDS-PAGE
- Top Product
- Discover our top product PPM1G 蛋白
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Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (AA 1-546) protein (GST tag)
PPM1G 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (AA 462-544) protein (GST tag)PPM1G 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (AA 1-546) protein (His tag)PPM1G 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PPM1G 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Transcript Variant 1) protein (His tag)PPM1G 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PPM1G 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl , 0.1 mM PMSF
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))
- 别名
- PPM1G (PPM1G 产品)
- 别名
- AI385652 Protein, Fin13 Protein, PP2CG Protein, PP2CGAMMA Protein, PPP2CG Protein, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent 1G Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1G Protein, Ppm1g Protein, PPM1G Protein
- 背景
- PPM1G, as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thr protein phosphatases that is negative regulators of cell stress response pathways. This enzyme localizes to the cytoplasm and is widely expressed, with most abundant expression detected in testis, skeletal muscle and heart. It is necessary for the dephosphorylation of pre-mRNA splicing factors, which is an important process for the formation of the functional spliceosome. Recombinant human PPM1G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 61.4 kDa (566aa)
- NCBI登录号
- NP_817092
- UniProt
- O15355
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