GNMT Protein (AA 1-295) (His tag)
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- 抗原 See all GNMT 蛋白
- GNMT (Glycine N-Methyltransferase (GNMT))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-295
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GNMT protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
- Top Product
- Discover our top product GNMT 蛋白
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Glycine N-Methyltransferase (GNMT) (AA 1-295) protein (His tag)
GNMT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Glycine N-Methyltransferase (GNMT) (AA 1-100) protein (GST tag)GNMT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glycine N-Methyltransferase (GNMT) (AA 1-295) protein (GST tag)GNMT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glycine N-Methyltransferase (GNMT) (AA 2-295) protein (His tag)Crystallography grade GNMT 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycine N-Methyltransferase (GNMT) (AA 2-293) protein (His tag)Crystallography grade GNMT 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycine N-Methyltransferase (GNMT) protein (Myc-DYKDDDDK Tag)GNMT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- GNMT (Glycine N-Methyltransferase (GNMT))
- 别名
- GNMT (GNMT 产品)
- 别名
- glycine N-methyltransferase Protein, CpipJ_CPIJ015956 Protein, Gnmt Protein, GNMT Protein
- 背景
- Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 34.9 kDa (315aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_061833
- UniProt
- Q14749
- 途径
- Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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