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UMPS Protein (AA 1-480) (His tag)
UMPS
宿主: 人
宿主: Baculovirus infected Insect Cells
Recombinant
> 95 % by SDS - PAGE
SDS
产品细节
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抗原
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UMPS
(Uridine Monophosphate Synthetase (UMPS))
蛋白类型
Recombinant
产品特性
AA 1-480
宿主
All origins for UMPS 蛋白
人
资源
All sources for UMPS 蛋白
Baculovirus infected Insect Cells
标记
This UMPS protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
序列
MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV HHHHHH
纯度
> 95 % by SDS - PAGE
内毒素水平
< 1.0 EU per 1ug of protein (determined by LAL method)
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
0.25 mg/mL
缓冲液
Liquid. In Phosphate Buffered Saline ( pH 7.4).
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
目标详细情况
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抗原
UMPS
(Uridine Monophosphate Synthetase (UMPS))
别名
UMPS (UMPS 产品 )
别名
zgc:55702 Protein, zgc:55723 Protein, UMPS Protein, OPRT Protein, 1700095D23Rik Protein, AA408257 Protein, AL033308 Protein, BB164745 Protein, Orotidine 5'-phosphate decarboxylase Protein, uridine monophosphate synthetase Protein, uridine monophosphate synthetase L homeolog Protein, uridine 5'-monophosphate synthase Protein, umps-1 Protein, umps Protein, umps.L Protein, UMPS Protein, LOC100556619 Protein, Umps Protein
背景
UMPS, also known as uridine 5'-monophosphate synthase, is a bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis. It is an indispensable component in this metabolic pathway and is a target for antimalarial and antitumor drugs. Its expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but It in the normal tissues can help predict the toxicities associated with 5-FU. Recombinant human UMPS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
分子量
53kDa (486aa) 50-70kDa (SDS-PAGE under reducing conditions)
NCBI登录号
NP_000364
UniProt
P11172
途径
Ribonucleoside Biosynthetic Process
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UMPS 蛋白
UMPS Protein (AA 1-480) (His tag) (ABIN6388096)