Cathepsin B Protein (CTSB) (AA 18-339) (His tag)
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- 抗原 See all Cathepsin B (CTSB) 蛋白
- Cathepsin B (CTSB)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 18-339
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This Cathepsin B protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- 序列
- HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH
- 纯度
- > 90% by SDS-PAGE
- 内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 2,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.
- Top Product
- Discover our top product CTSB 蛋白
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- 抗原
- Cathepsin B (CTSB)
- 别名
- Cathepsin B (CTSB 产品)
- 背景
- CTSB, also known as cathepsin B preproprotein, is a papain-family cysteine protease that is normally located in lysosomes. It has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Recombinant mouse CTSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 36.4 kDa (330aa)
- NCBI登录号
- NP_031824
- UniProt
- P10605
- 途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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