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BTD Protein (AA 44-545) (His tag)
BTD
宿主: 人
宿主: Baculovirus infected Insect Cells
Recombinant
> 85 % by SDS - PAGE
SDS
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抗原
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BTD
(Biotinidase (BTD))
蛋白类型
Recombinant
产品特性
AA 44-545
宿主
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人
资源
All sources for BTD 蛋白
Baculovirus infected Insect Cells
标记
This BTD protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
序列
AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH
纯度
> 85 % by SDS - PAGE
内毒素水平
< 1.0 EU per 1 microgram of protein (determined by LAL method)
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
0.25 mg/mL
缓冲液
Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
目标详细情况
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抗原
BTD
(Biotinidase (BTD))
别名
BTD (BTD 产品 )
别名
BTD Protein, CG3599 Protein, CT12113 Protein, Dmel\\CG3599 Protein, zgc:153333 Protein, Biotinase Protein, Biotinidase Protein, biotinidase Protein, Btnd Protein, btd Protein, BTD Protein, Btd Protein
背景
BTD, also known as biotinidase isoform 1, is a member of the nitrilase superfamily, which consists of 12 families of nitrilases, amidases, carbamylases, and N-acyltrasferases. It catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. It may have an important regulatory role in chromatin/DNA function. Recombinant human BTD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
分子量
57.8kDa (510aa) 50-70kDa (SDS-PAGE under reducing conditions)
NCBI登录号
NP_001268652
UniProt
P43251
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BTD 蛋白
BTD Protein (AA 44-545) (His tag) (ABIN6387911)