BTD Protein (His tag)
Our Local Distributor
北京 101111
Quick Overview for BTD Protein (His tag) (ABIN7194393)
抗原
See all BTD 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
标记
- This BTD protein is labelled with His tag.
-
原理
- Recombinant Human Biotinidase/BTD Protein (His Tag)
-
序列
- Met 1-Asp543
-
产品特性
- A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is > 500pmol/min/μg.
-
-
Biotinidase (BTD) (AA 1-543) protein (GST tag)
BTD 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Biotinidase (BTD) (AA 44-545) protein (His tag)BTD 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 85 % SDS
Biotinidase (BTD) protein (Myc-DYKDDDDK Tag)BTD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- BTD (Biotinidase (BTD))
-
别名
- Biotinidase/BTD
-
背景
- BTD,Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing offats,carbohydrates, andproteins. Biotin is attached to these carboxylase enzymes through anamino acid(the building material of proteins) calledlysine, forming a complex calledbiocytin.
-
分子量
-
Calculated MW: 58.2 kDa
Observed MW: 66-76 kDa
-
基因ID
- 686
-
UniProt
- P43251
抗原
-
