POLD4 Protein (AA 1-107) (His tag)
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- 抗原 See all POLD4 蛋白
- POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-107
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This POLD4 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLD4 蛋白
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Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-107) protein (His tag)
POLD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-34) protein (GST tag)POLD4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) protein (Myc-DYKDDDDK Tag)POLD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))
- 别名
- POLD4 (POLD4 产品)
- 别名
- 2410012M21Rik Protein, AI463381 Protein, AW060307 Protein, Polds Protein, p12 Protein, POLDS Protein, DNA polymerase delta 4, accessory subunit Protein, polymerase (DNA-directed), delta 4 Protein, POLD4 Protein, Pold4 Protein
- 背景
- POLD4 is the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human POLD4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 14.8 kDa (130aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_066996
- UniProt
- Q9HCU8
- 途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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