TIMD4 Protein (Fc Tag,Biotin)
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- 抗原 See all TIMD4 蛋白
- TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))
- 蛋白类型
- Recombinant
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宿主
- 人, 小鼠
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资源
- CHO Cells
- 标记
- This TIMD4 protein is labelled with Fc Tag,Biotin.
- 原理
- Tim-4 (mouse):Fc (human) (rec.) (Biotin)
- 特异性
- The extracellular domain of mouse Tim-4 (aa 22-279) is fused to the N-terminus of the Fc region of human IgG1.
- 产品特性
- Protein. The extracellular domain of mouse Tim-4 (aa 22-279) is fused to the N-terminus of the Fc region of human IgG1. Source: CHO cells. Endotoxin content: <0.05EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). Extracellular vesicles (EVs) are released by a variety of cells into the cellular microenvironment and have the natural ability of delivering different cargos and carry bioactive molecules such as non-coding RNA, miRNAs, genomic DNA, lipids, growth factors and signaling molecules. EVs can be divided into exosomes (30-100nm), microvesicles (100-1000nm) and apoptotic bodies (>1000nm). EVs play substantial roles not only in the regulation of normal physiological processes but also in disease pathogenesis and their cargo reflects the status of parental cells at the time of secretion. Various studies are currently being conducted to develop therapeutic and diagnostic methods targeting or utilizing EVs. Therefore, developing ideal methods for isolating and quantifying EVs is an active area of research. EVs express phosphatidylserine (PS) on their outer lipid bilayer. Tim-4 (T cell immunoglobulin and mucin domain-containing protein 4) is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and TIM family. Tim-4 contains one Ig-like V-type (immunoglobulin-like) domain. It is expressed on dendritic cells and macrophages. Tim-4 plays an important role in the proliferation of T helper type 2 (Th2) cells. Tim-4 binds to phosphatidylserine (PS) on the surface of apoptotic cells in a calcium-dependent manner and mediates phagocytosis of apoptotic cells. EV membranes are rich in phosphatidylserine (PS) and Tim-4 binds to PS on the surface of EVs. A new protocol from the group of Prof. Rikinari Hanayama describes an affinity-based method for isolating EVs using streptavidin magnetic beads conjugated with Tim-4-biotin to capture EVs in a calcium-dependent manner. This new protocol could replace ultracentrifugation, that is the most commonly used method for purifying EVs. This new Tim-4-dependent method gives good yield, high purity and allows isolation of all populations of EVs compared to other approaches (ultracentrifugation, PEG precipitation or selected antibodies immunoprecipitation). See Reference 2 for a complete protocol (Download available).
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.05EU/μg protein (LAL test, Lonza).
- Biological Activity Comment
- Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The Tim-4 (mouse):Fc (human) (rec.) (Biotin) is used with magnetic beads to isolate extracellular vesicles. 120ng of the protein is sufficient to isolate 1010 particles in a calcium-dependent manner.
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- Discover our top product TIMD4 蛋白
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T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4) (AA 25-314) protein (His tag)
TIMD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4) (AA 25-305) protein (His tag)TIMD4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4) protein (His tag)TIMD4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 94 % as determined by SDS-PAGE
T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4) (AA 25-314) protein (His tag)Crystallography grade TIMD4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4) (AA 23-279) protein (His tag)Crystallography grade TIMD4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4) (AA 25-378) protein (rho-1D4 tag)Crystallography grade TIMD4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4) (AA 23-343) protein (rho-1D4 tag)Crystallography grade TIMD4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from 0.2μm-filtered solution in PBS.
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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- 抗原
- TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))
- 别名
- Tim-4 (TIMD4 产品)
- 别名
- RGD1564516 Protein, MGC99322 Protein, TIMD4 Protein, B430010N18Rik Protein, TIM-4 Protein, Tim4 Protein, SMUCKLER Protein, TIM4 Protein, T-cell immunoglobulin and mucin domain containing 4 Protein, T-cell immunoglobulin and mucin domain containing 4 L homeolog Protein, T cell immunoglobulin and mucin domain containing 4 Protein, Timd4 Protein, TIMD4 Protein, timd4.L Protein
- 背景
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Alternate Names/Synonyms: TIM4, TIMD4, T Cell Immunoglobulin and Mucin Domain-containing Protein 4
Product Description: Extracellular vesicles (EVs) are released by a variety of cells into the cellular microenvironment and have the natural ability of delivering different cargos and carry bioactive molecules such as non-coding RNA, miRNAs, genomic DNA, lipids, growth factors and signaling molecules. EVs can be divided into exosomes (30-100nm), microvesicles (100-1000nm) and apoptotic bodies (>1000nm). EVs play substantial roles not only in the regulation of normal physiological processes but also in disease pathogenesis and their cargo reflects the status of parental cells at the time of secretion. Various studies are currently being conducted to develop therapeutic and diagnostic methods targeting or utilizing EVs. Therefore, developing ideal methods for isolating and quantifying EVs is an active area of research. EVs express phosphatidylserine (PS) on their outer lipid bilayer. Tim-4 (T cell immunoglobulin and mucin domain-containing protein 4) is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and TIM family. Tim-4 contains one Ig-like V-type (immunoglobulin-like) domain. It is expressed on dendritic cells and macrophages. Tim-4 plays an important role in the proliferation of T helper type 2 (Th2) cells. Tim-4 binds to phosphatidylserine (PS) on the surface of apoptotic cells in a calcium-dependent manner and mediates phagocytosis of apoptotic cells. EV membranes are rich in phosphatidylserine (PS) and Tim-4 binds to PS on the surface of EVs. A new protocol from the group of Prof. Rikinari Hanayama describes an affinity-based method for isolating EVs using streptavidin magnetic beads conjugated with Tim-4-biotin to capture EVs in a calcium-dependent manner. This new protocol could replace ultracentrifugation, that is the most commonly used method for purifying EVs. This new Tim-4-dependent method gives good yield, high purity and allows isolation of all populations of EVs compared to other approaches (ultracentrifugation, PEG precipitation or selected antibodies immunoprecipitation). See Reference 2 for a complete protocol (Download available).
- NCBI登录号
- NP_848874
- 途径
- Cancer Immune Checkpoints
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