CD276 Protein (CD276) (AA 47-219) (His tag)
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- 抗原 See all CD276 蛋白
- CD276
- 生物活性
- Active
- 产品特性
- AA 47-219
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CD276 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Cluster Of Differentiation 276 (CD276) also known as B7-H3 in human, is a member of the B7/CD28 superfamily of costimulatory molecules serving as an accessory modulator of T-cell response. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production. Besides, Sialic acid-binding Ig-like lectin 12 (SIGLEC12) has been identified as an interactor of CD276, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD276 and recombinant human SIGLEC12. Briefly, CD276 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIGLEC12-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD276 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CD276 and SIGLEC12 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CD276 with SIGLEC12.
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- Discover our top product CD276 蛋白
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CD276 (CD276) (AA 29-245) protein (Fc Tag)
CD276 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-245) protein (His tag)CD276 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 27-461) protein (Fc Tag)CD276 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-465) protein (His tag)CD276 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-245) protein (His-Avi Tag,Biotin)CD276 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) (AA 29-248) protein (His tag)CD276 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD276 (CD276) protein (mFc-His Tag)CD276 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD276 (CD276) (AA 28-140) protein (His-DYKDDDDK-Strep II Tag)CD276 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 28-238) protein (His-DYKDDDDK-Strep II Tag)CD276 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD276 (CD276) (AA 237-461) protein (His-DYKDDDDK-Strep II Tag)CD276 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
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- 应用备注
- Isoelectric Point: 5.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- CD276
- 别名
- Cluster Of Differentiation 276 (CD276) (CD276 产品)
- 别名
- sc:d0645 Protein, zgc:158279 Protein, 6030411F23Rik Protein, AU016588 Protein, B7RP-2 Protein, B7h3 Protein, B7H3 Protein, 4Ig-B7-H3 Protein, B7-H3 Protein, CD276 molecule Protein, CD276 molecule S homeolog Protein, CD276 antigen Protein, Cd276 molecule Protein, CD276 Protein, cd276.S Protein, cd276 Protein, Cd276 Protein
- 背景
- Alternative Names: B7-H3, B7H3, CD276 Molecule, B7 homolog 3, Costimulatory molecule
- 分子量
- 22kDa
- UniProt
- Q5ZPR3
- 途径
- Cancer Immune Checkpoints
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