SOCS3 Protein (AA 1-225) (His tag)
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- 抗原 See all SOCS3 蛋白
- SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))
- 生物活性
- Active
- 产品特性
- AA 1-225
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SOCS3 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- Suppressor of cytokine signaling 3 (SOCS3) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family that negatively regulates cytokine signal transduction. SOCS3 is feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. SOCS3 also can bind to JAK2 kinase inhibits its kinase activity and nhibits insulin signaling in adipose tissue and the liver. Besides, Glycoprotein 130 (gp130) has been identified as an interactor of SOCS3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SOCS3 and recombinant mouse gp130. Briefly, SOCS3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SOCS3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SOCS3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of SOCS3 and gp130 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of SOCS3 with gp130.
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- Discover our top product SOCS3 蛋白
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Suppressor of Cytokine Signaling 3 (SOCS3) (AA 1-225) protein (GST tag)
SOCS3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Suppressor of Cytokine Signaling 3 (SOCS3) (AA 1-225) protein (His-IF2DI Tag)SOCS3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Suppressor of Cytokine Signaling 3 (SOCS3) (AA 1-225), (full length) protein (His tag)SOCS3 宿主: 大鼠 宿主: 酵母菌 Recombinant > 90 % SDS
Suppressor of Cytokine Signaling 3 (SOCS3) (AA 1-225), (full length) protein (His-SUMO Tag)SOCS3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
- Isoelectric Point: 8.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))
- 别名
- Suppressors Of Cytokine Signaling 3 (SOCS3) (SOCS3 产品)
- 别名
- socs3 Protein, fj09e12 Protein, zgc:56537 Protein, wu:fj09e12 Protein, MGC64393 Protein, MGC75749 Protein, xsocs3 Protein, MGC81248 Protein, atod4 Protein, cis3 Protein, cish3 Protein, socs-3 Protein, ssi-3 Protein, ssi3 Protein, SOCS3 Protein, ATOD4 Protein, CIS3 Protein, Cish3 Protein, SOCS-3 Protein, SSI-3 Protein, SSI3 Protein, Cis3 Protein, EF-10 Protein, Ef10 Protein, Ssi3 Protein, Socs-3 Protein, Ssi-3 Protein, suppressor of cytokine signaling 3a Protein, suppressor of cytokine signaling 3 S homeolog Protein, suppressor of cytokine signaling 3 Protein, suppressor of cytokine signaling 3 L homeolog Protein, socs3a Protein, socs3.S Protein, socs3 Protein, socs3.L Protein, SOCS3 Protein, Socs3 Protein
- 背景
- Alternative Names: ATOD4, CIS3, Cish3, MGC71791, SOCS-3, SSI-3, SSI3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3
- 分子量
- 26kDa
- UniProt
- O35718
- 途径
- JAK/STAT Signaling, Response to Growth Hormone Stimulus, Hepatitis C
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