WNT5A Protein (AA 62-380) (GST tag)
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- 抗原 See all WNT5A 蛋白
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- 生物活性
- Active
- 产品特性
- AA 62-380
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This WNT5A protein is labelled with GST tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal GST Tag
- 纯度
- > 95 %
- Biological Activity Comment
- Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) is a ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Besides, WNT Inhibitory Factor 1 (WIF1) has been identified as an interactor of WNT5A, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse WNT5A and recombinant rat WIF1. Briefly, WNT5A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to WIF1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-WNT5A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of WNT5A and WIF1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of WNT5A with WIF1.
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Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 1-380) protein (GST tag)
WNT5A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 201-300) protein (GST tag)WNT5A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 62-380) protein (His tag)Crystallography grade WNT5A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) protein (Myc-DYKDDDDK Tag)WNT5A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 8.8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- 别名
- Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) (WNT5A 产品)
- 别名
- hWNT5A Protein, wnt-5A Protein, wnt5a-B Protein, WNT5A Protein, 8030457G12Rik Protein, Wnt-5a Protein, Wnt family member 5A Protein, Wnt family member 5A S homeolog Protein, wingless-type MMTV integration site family, member 5a Protein, wingless-type MMTV integration site family, member 5A Protein, wingless-related MMTV integration site 5A Protein, Wnt signaling ligand Protein, WNT5A Protein, Wnt5a Protein, wnt5a.S Protein, wnt5a Protein, LOC578814 Protein, wnt5 Protein
- 分子量
- 62kDa
- 途径
- WNT signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation
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