WNT5A Protein (AA 38-380)
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- 抗原 See all WNT5A 蛋白
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 38-380
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宿主
- 小鼠, 人
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资源
- Mammalian Cells
- 应用范围
- Functional Studies (Func)
- 特异性
- Stem cell or organoid culture
- 纯化方法
- This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
- 纯度
- 70-75 %
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg)
- Biological Activity Comment
- The inhibitory activity of Wnt5a on the canonical Wnt pathway has been measured using TCF-based Wnt reporter stable cell line stimulated by mouse Wnt3a. IE50 of Wnt5a is about 50 - 100 ng/mL in the presence of 1 -2 ng/mL of mouse Wnt3a.
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- Discover our top product WNT5A 蛋白
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Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 1-380) protein (GST tag)
WNT5A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 201-300) protein (GST tag)WNT5A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 62-380) protein (His tag)Crystallography grade WNT5A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 62-380) (Active) protein (GST tag)WNT5A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) protein (Myc-DYKDDDDK Tag)WNT5A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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100 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
- 注意事项
- Mix the protein by pipetting up and down but not by vortexing
- 储存条件
- -20 °C
- 储存方法
- Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
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- 抗原
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- 别名
- Wnt5a (WNT5A 产品)
- 别名
- hWNT5A Protein, wnt-5A Protein, wnt5a-B Protein, WNT5A Protein, 8030457G12Rik Protein, Wnt-5a Protein, Wnt family member 5A Protein, Wnt family member 5A S homeolog Protein, wingless-type MMTV integration site family, member 5a Protein, wingless-type MMTV integration site family, member 5A Protein, wingless-related MMTV integration site 5A Protein, Wnt signaling ligand Protein, WNT5A Protein, Wnt5a Protein, wnt5a.S Protein, wnt5a Protein, LOC578814 Protein, wnt5 Protein
- 背景
- The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. Protein Wnt5a is a protein that is encoded by the WNT5A gene. This gene is a member of the noncanonical WNT gene family. Mature mouse Wnt5a is 100 % identical in amino acids to mature human Wnt5a after being secreted from expression cells. Wnt5a protein can inhibit or activate TCF-based Wnt signaling.
- 分子量
- 45 kDa evaluated by SDS-PAGE under reducing conditions
- 基因ID
- 7474, 22418
- NCBI登录号
- NC_000080
- UniProt
- P41221, P22725
- 途径
- WNT signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation
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