IL1R1 Protein (AA 21-109) (His tag)
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- 抗原 See all IL1R1 蛋白
- IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
- 生物活性
- Active
- 产品特性
- AA 21-109
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IL1R1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1a), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Thus, a binding ELISA assay was conducted to detect the association of IL1R1 with IL1a. Briefly, IL1R1 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1a was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL1R1 with IL1a.
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Interleukin 1 Receptor, Type I (IL1R1) (AA 20-338) protein (His tag)
IL1R1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 1 Receptor, Type I (IL1R1) (AA 18-332) protein (His tag)IL1R1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 1 Receptor, Type I (IL1R1) protein (Fc Tag)IL1R1 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE
Interleukin 1 Receptor, Type I (IL1R1) (AA 19-328) proteinIL1R1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Interleukin 1 Receptor, Type I (IL1R1) (AA 18-127) protein (GST tag)IL1R1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 1 Receptor, Type I (IL1R1) (Active) protein (Fc Tag)IL1R1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
Interleukin 1 Receptor, Type I (IL1R1) (Active) protein (His tag,Fc Tag)IL1R1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Interleukin 1 Receptor, Type I (IL1R1) (Active) protein (His tag)IL1R1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 1 Receptor, Type I (IL1R1) (Active) protein (His tag)IL1R1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 1 Receptor, Type I (IL1R1) (AA 357-569) protein (His tag)Crystallography grade IL1R1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Isoelectric Point: 7.8
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
- 别名
- Interleukin 1 Receptor Type I (IL1R1) (IL1R1 产品)
- 别名
- CD121A Protein, D2S1473 Protein, IL-1R-alpha Protein, IL1R Protein, IL1RA Protein, P80 Protein, CD121a Protein, CD121b Protein, IL-iR Protein, Il1r-1 Protein, DER4 Protein, Fit-1 Protein, Ly84 Protein, ST2L Protein, St2 Protein, St2-rs1 Protein, T1 Protein, T1/ST2 Protein, interleukin 1 receptor type 1 Protein, interleukin 1 receptor, type I Protein, interleukin 1 receptor-like 1 Protein, IL1R1 Protein, Il1r1 Protein, Il1rl1 Protein
- 背景
- Alternative Names: CD121a, CD121-A, IL1-R1, IL1R, IL1RA, P80, CD121 Antigen-Like Family Member A, Interleukin-1 Receptor Alpha
- 分子量
- 14kDa
- UniProt
- P14778
- 途径
- NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints
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