MASP2 Protein (AA 445-683) (His tag)
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- 抗原 See all MASP2 蛋白
- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
- 生物活性
- Active
- 产品特性
- AA 445-683
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MASP2 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- MASP2 (Mannan-binding lectin serine protease 2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. The preproprotein of MASP2 is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease, which is able to associate with MBL2. Thus, a functional binding ELISA assay was constructed to detect the association of rhMASP2 with MBL2. Briefly, rhMASP-2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MBL2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MASP-2 pAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MASP2 with MBL2 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of MASP2 with MBL2.
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- Discover our top product MASP2 蛋白
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Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 288-686) protein (His tag)
MASP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-686) protein (His tag)MASP2 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag,Biotin)MASP2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 1-686) protein (GST tag)MASP2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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- 应用备注
- Isoelectric Point: 5.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
- 别名
- Mannose Associated Serine Protease 2 (MASP2) (MASP2 产品)
- 别名
- MAP19 Protein, MASP-2 Protein, MASP1P1 Protein, sMAP Protein, MAp19 Protein, mannan binding lectin serine peptidase 2 Protein, mannan-binding lectin serine peptidase 2 Protein, MASP2 Protein, Masp2 Protein
- 背景
- Alternative Names: Mannose-binding protein-associated serine protease 2, mannan-Binding Lectin Serine Peptidase 2, MBL-associated serine protease 2
- 分子量
- 27kDa
- UniProt
- O00187
- 途径
- Complement System
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