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VEGFC Protein (AA 47-413) (His tag)

VEGFC 宿主: 人 宿主: 大肠杆菌(E. Coli) > 90 % CC, AcA Active
产品编号 ABIN6239783
发货至: 中国
  • 抗原 See all VEGFC 蛋白
    VEGFC (Vascular Endothelial Growth Factor C (VEGFC))
    生物活性
    Active
    产品特性
    AA 47-413
    宿主
    • 15
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    资源
    • 9
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    大肠杆菌(E. Coli)
    标记
    This VEGFC protein is labelled with His tag.
    应用范围
    Cell Culture (CC), Activity Assay (AcA)
    产品特性
    Tag location: N-terminal His Tag
    纯度
    > 90 %
    Biological Activity Comment
    Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. The dose-effect curve of VEGFC was shown in Figure 1. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339μg/mL The dose-effect curve of VEGFC on ECV304 cells
    Top Product
    Discover our top product VEGFC 蛋白
  • 应用备注
    Isoelectric Point: 8.2
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    储存液
    Dithiothreitol (DTT), Other preservative, ProClin
    注意事项
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • 抗原
    VEGFC (Vascular Endothelial Growth Factor C (VEGFC))
    Abstract
    VEGFC 产品
    别名
    VEGFC Protein, vegf-c Protein, xvegf-c Protein, Flt4-L Protein, VRP Protein, AW228853 Protein, VEGF-C Protein, vascular endothelial growth factor c Protein, vascular endothelial growth factor C Protein, vascular endothelial growth factor C S homeolog Protein, vegfc Protein, VEGFC Protein, vegfc.S Protein, Vegfc Protein
    背景
    Alternative Names: VEGF-C, Flt4-L, VRPL, Vascular Endothelial Growth Factor-Related Protein
    分子量
    45kDa
    UniProt
    P49767
    途径
    RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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