TNFRSF12A Protein (AA 24-126) (His tag,GST tag)
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- 抗原 See all TNFRSF12A 蛋白
- TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
- 生物活性
- Active
- 产品特性
- AA 24-126
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This TNFRSF12A protein is labelled with His tag,GST tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His and GST Tag
- 纯度
- > 80 %
- Biological Activity Comment
- TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TNFRSF12A with TNFa.
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Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (Active) protein
TNFRSF12A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (AA 28-80) protein (Fc Tag)TNFRSF12A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (AA 28-80) protein (Fc Tag)TNFRSF12A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (AA 28-80) protein (His tag)TNFRSF12A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) protein (mFc Tag)TNFRSF12A 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (AA 28-129) protein (GST tag)TNFRSF12A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (AA 28-129) protein (rho-1D4 tag)Crystallography grade TNFRSF12A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (AA 28-129) protein (rho-1D4 tag)Crystallography grade TNFRSF12A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 6.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
- Abstract
- TNFRSF12A 产品
- 别名
- CD266 Protein, FN14 Protein, TWEAKR Protein, AI255180 Protein, C87282 Protein, Fn14 Protein, HPIP Protein, TWEAK-R Protein, TweakR Protein, TNF receptor superfamily member 12A Protein, tumor necrosis factor receptor superfamily, member 12a Protein, TNFRSF12A Protein, Tnfrsf12a Protein
- 背景
- Alternative Names: CD266, TNFRSF12-A, FN14, TWEAKR, Tumour Necrosis Factor Related Weak Inducer Of Apoptosis, Fibroblast growth factor-inducible immediate-early response protein 14
- 分子量
- 44kDa
- UniProt
- Q9NP84
- 途径
- Apoptosis, Regulation of Cell Size
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