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IL17C Protein (AA 36-187) (His tag)

IL17C 宿主: 人 宿主: 大肠杆菌(E. Coli) > 95 % AcA, CC Active
产品编号 ABIN6239767
发货至: 中国
  • 抗原 See all IL17C 蛋白
    IL17C (Interleukin 17C (IL17C))
    生物活性
    Active
    产品特性
    AA 36-187
    宿主
    • 9
    • 4
    • 2
    • 1
    资源
    • 7
    • 5
    • 2
    • 1
    大肠杆菌(E. Coli)
    标记
    This IL17C protein is labelled with His tag.
    应用范围
    Activity Assay (AcA), Cell Culture (CC)
    产品特性
    Tag location: N-terminal His Tag
    纯度
    > 95 %
    Biological Activity Comment
    IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to plays a crucial role in innate immunity of the epithelium. IL17C binds to IL17RA with affinity. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17C with recombinant human IL17RA. Briefly, IL17C were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17C with IL17RA.
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    Discover our top product IL17C 蛋白
  • 应用备注
    Isoelectric Point: 8.6
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    储存液
    Dithiothreitol (DTT), Other preservative, ProClin
    注意事项
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • 抗原
    IL17C (Interleukin 17C (IL17C))
    Abstract
    IL17C 产品
    别名
    IL17C Protein, il17-3 Protein, CX2 Protein, IL-17C Protein, IL-21 Protein, interleukin 17C Protein, interleukin 17c Protein, IL17C Protein, il17c Protein, Il17c Protein
    背景
    Alternative Names: Cytokine CX2
    分子量
    19kDa
    UniProt
    Q9P0M4
    途径
    Cellular Response to Molecule of Bacterial Origin
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