XRCC5 Protein (AA 251-455, partial) (His tag)
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- 抗原 See all XRCC5 蛋白
- XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))
- 蛋白类型
- Recombinant
- 产品特性
- partial, AA 251-455
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This XRCC5 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- LTIGSNLSIR IAAYKSILQE RVKKTWTVVD AKTLKKEDIQ KETVYCLNDD DETEVLKEDI IQGFRYGSDI VPFSKVDEEQ MKYKSEGKCF SVLGFCKSSQ VQRRFFMGNQ VLKVFAARDD EAAAVALSSL IHALDDLDMV AIVRYAYDKR ANPQVGVAFP HIKHNYECLV YVQLPFMEDL RQYMFSSLKN SKKYAPTEAQ LNAVD
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product XRCC5 蛋白
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X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5) (AA 1-732) protein (GST tag)
XRCC5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5) (AA 2-732) protein (His tag)Crystallography grade XRCC5 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))
- 别名
- XRCC5 (XRCC5 产品)
- 别名
- AI314015 Protein, Ku80 Protein, Ku86 Protein, Kup80 Protein, KARP-1 Protein, KARP1 Protein, KU80 Protein, KUB2 Protein, NFIV Protein, xrcc5-a Protein, XRCC5 Protein, X-ray repair cross complementing 5 Protein, X-ray repair complementing defective repair in Chinese hamster cells 5 Protein, X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog Protein, XRCC5 Protein, Xrcc5 Protein, xrcc5.L Protein
- 背景
- Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing th together. The assbly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
- 分子量
- 27.53 kDa
- UniProt
- P13010
- 途径
- DNA Damage Repair
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