Parkin Protein (AA 1-465, full length) (His-SUMO Tag)
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- 抗原 See all Parkin (PARK2) 蛋白
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
- 蛋白类型
- Recombinant
- 产品特性
- full length, AA 1-465
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Parkin protein is labelled with His-SUMO Tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MIVFVRFNSS HGFPVEVDSD TSIFQLKEVV AKRQGVPADQ LRVIFAGKEL RNDWTVQNCD LDQQSIVHIV QRPWRKGQEM NATGGDDPRN AAGGCEREPQ SLTRVDLSSS VLPGDSVGLA VILHTDSRKD SPPAGSPAGR SIYNSFYVYC KGPCQRVQPG KLRVQCSTCR QATLTLTQGP SCWDDVLIPN RMSGECQSPH CPGTSAEFFF KCGAHPTSDK ETSVALHLIA TNSRNITCIT CTDVRSPVLV FQCNSRHVIC LDCFHLYCVT RLNDRQFVHD PQLGYSLPCV AGCPNSLIKE LHHFRILGEE QYNRYQQYGA EECVLQMGGV LCPRPGCGAG LLPEPDQRKV TCEGGNGLGC GFAFCRECKE AYHEGECSAV FEASGTTTQA YRVDERAAEQ ARWEAASKET IKKTTKPCPR CHVPVEKNGG CMHMKCPQPQ CRLEWCWNCG CEWNRVCMGD HWFDV
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product PARK2 蛋白
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Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-165) protein (His-IF2DI Tag)
PARK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-387) protein (GST tag)PARK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 288-387) protein (GST tag)PARK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-465) protein (Strep Tag)Crystallography grade PARK2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-464) protein (Strep Tag)Crystallography grade PARK2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-465) protein (His tag)PARK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % by SDS - PAGE SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
- 别名
- PRKN2 (PARK2 产品)
- 别名
- CG10523 Protein, Dmel\\CG10523 Protein, Dpark Protein, SD01679 Protein, dpk Protein, AR-JP Protein, LPRS2 Protein, PDJ Protein, PRKN Protein, Park Protein, Prkn Protein, si:ch211-123f21.1 Protein, zgc:112390 Protein, pdr-1 Protein, PARK2 Protein, parkin Protein, parkin RBR E3 ubiquitin protein ligase Protein, Parkinson disease (autosomal recessive, juvenile) 2, parkin Protein, park Protein, PRKN Protein, Prkn Protein, prkn Protein, CpipJ_CPIJ014867 Protein, Park2 Protein
- 背景
- Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2 . Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context . Participates in the roval and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation . Mediates 'Lys-63'-linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation . Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy . Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1 and USP30 . Preferentially assbles 'Lys-6'-, 'Lys-11'- and 'Lys-63'-linked polyubiquitin chains following mitochondrial damage, leading to mitophagy . Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome, possibly playing a role in the regulation of neuron death . Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress . Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53 . May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity . May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene
- 分子量
- 67.6 kDa
- UniProt
- O60260
- 途径
- Autophagy, Ubiquitin Proteasome Pathway
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