HVEM (TNFRSF14)
(Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
Hi-5 Cells
标记
This HVEM protein is labelled with Fc Tag.
应用范围
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human TNFRSF14 / HVEM protein expressed in Hi-5 Insect cells.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by its ability to neutralize 0.25 ng/ml of hTNF&beta induced cytotoxicity on murine L929 cells. The expected ED50 for this effect is 1.3-1.9 ug/mL of HVEM-Fc.
TNFRSF14
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by SDS-PAGE
Active
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原
HVEM (TNFRSF14)
(Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.