HVEM Protein (AA 39-202) (hFc Tag)
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Quick Overview for HVEM Protein (AA 39-202) (hFc Tag) (ABIN7274832)
抗原
See all HVEM (TNFRSF14) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 39-202
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标记
- This HVEM protein is labelled with hFc Tag.
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原理
- Human HVEM/TNFRSF14 Protein
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序列
- Leu39-Val202
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产品特性
- Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu39-Val202.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200).,Immobilized Human BTLA, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5 ng/ml determined by ELISA (QC Test).,Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA.,Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) protein (His tag)
TNFRSF14 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) (Active) protein (His tag)TNFRSF14 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Func Active
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 38-200) protein (His-DYKDDDDK-Strep II Tag)TNFRSF14 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) (Active) protein (Fc Tag)TNFRSF14 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (Active) protein (Fc Tag)TNFRSF14 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) protein (His tag)TNFRSF14 宿主: 猕猴 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) protein (Fc Tag)TNFRSF14 宿主: 猕猴 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 38-283) protein (GST tag)TNFRSF14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (Active) protein (His tag,Fc Tag)TNFRSF14 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) protein (His tag)TNFRSF14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
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别名
- HVEM
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物质类
- Viral Protein
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背景
- Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
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分子量
- 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
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UniProt
- Q92956
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途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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