HGF Protein (Transcript Variant 1)
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- 抗原 See all HGF 蛋白
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- Hi-5 Cells
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human Hepatocyte growth factor / HGF (transcript variant 1) protein expressed in Hi-5 insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.
- Top Product
- Discover our top product HGF 蛋白
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Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) protein (His tag)
HGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) protein (Fc Tag)HGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-494), (AA 495-728) (Active) proteinHGF 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-124) protein (GST tag)HGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 477-728) protein (His tag)HGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 496-728) protein (His-IF2DI Tag)HGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 493-721) protein (His-IF2DI Tag)HGF 宿主: 小鸡 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 619-728) protein (GST tag)HGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
- 别名
- Hepatocyte growth factor / HGF (HGF 产品)
- 别名
- DFNB39 Protein, F-TCF Protein, HGFB Protein, HPTA Protein, SF Protein, HGF Protein, C230052L06Rik Protein, HGF/SF Protein, NK1 Protein, NK2 Protein, SF/HGF Protein, hepatocyte growth factor Protein, HGF Protein, Hgf Protein
- 背景
- This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
- 分子量
- 83.13
- NCBI登录号
- NP_000592
- 途径
- RTK signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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