HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
蛋白类型
Recombinant
生物活性
Active
产品特性
AA 32-494, AA 495-728
宿主
人
资源
CHO Cells
产品特性
ED50 < 10 ng/mL, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1x10^5 units/mg. Alpha chain: AA 32-494, beta chain: AA 495-728
Crystallography grade
HGF
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O or PBS at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant humanHepatocyte Growth Factor (HGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, human Hepatocyte Growth Factor (HGF) should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
有效期
6 months
抗原
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.Recombinant human Hepatocyte Growth Factor (rhHGF) is produced in CHO cells and consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in the formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues. A fully biologically active molecule, rhHGF has a molecular mass of around 88-90 kDa analyzed by reducing SDS-PAGE. Synonyms: