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CD27 Protein (AA 21-192) (Fc Tag,AVI tag,Biotin)

CD27 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
产品编号 ABIN4949012
发货至: 中国
  • 抗原 See all CD27 蛋白
    CD27 (CD27 Molecule (CD27))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 21-192
    宿主
    • 21
    • 11
    • 3
    • 3
    • 1
    资源
    • 17
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    HEK-293 Cells
    标记
    This CD27 protein is labelled with Fc Tag,AVI tag,Biotin.
    应用范围
    Functional Studies (Func)
    品牌
    MABSol®,PrecisionAvi
    序列
    AA 21-192
    特异性
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    产品特性
    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    纯度
    >95 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product CD27 蛋白
  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    Tris with Glycine, Arginine and NaCl,  pH 7.5
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
  • 抗原
    CD27 (CD27 Molecule (CD27))
    别名
    CD27 (CD27 产品)
    别名
    TNFRSF7 Protein, S152 Protein, Tnfrsf7 Protein, Tp55 Protein, S152. LPFS2 Protein, T14 Protein, CD27 molecule Protein, CD27 antigen Protein, CD27 Protein, Cd27 Protein
    背景
    CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
    分子量
    47.7 kDa
    NCBI登录号
    NP_001233
    途径
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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